Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Oxidative stress mediates end organ damage in a novel model of acetaminophen toxicity in Drosophila Scientific Reports USMLE Step 2 CK Question of the Day: Altered Mental State After Over the counter pain reliever and Prescription Hydrocodone acetaminophen Osmosis Blog 05. Acetaminophen Toxicity Hospital Handbook